| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kolessar, Ashley O (10)
|
MAUN-3 |
59.01 |
59.48 |
0.47 |
| 2 |
Waslyn, Rylee E (10)
|
MAWAC |
1:03.01 |
1:01.71 |
-1.30 |
| 3 |
Szerencsits, Olivia S (10)
|
MAPAAC |
1:04.38 |
1:03.14 |
-1.24 |
| 4 |
Brandt, Mackenzie A (10)
|
MAWAC |
1:07.36 |
1:04.63 |
-2.73 |
| 5 |
Evans, Olivia M (10)
|
MASEA |
1:09.21 |
1:05.36 |
-3.85 |
| 6 |
Saul, Yuran A (10)
|
MAJRAY |
1:06.28 |
1:06.59 |
0.31 |
| 7 |
Turco, Lily M (10)
|
MAGRA |
1:07.31 |
1:06.69 |
-0.62 |
| 8 |
Wilson, Mekhi S (10)
|
MASSC |
1:06.20 |
1:06.89 |
0.69 |
| 9 |
Agne, Kira W (9)
|
MAWAC |
1:07.59 |
1:07.54 |
-0.05 |
| 10 |
McNulty, Mary Frances T (10)
|
MACBST |
1:09.54 |
1:08.47 |
-1.07 |
| 11 |
Lunn, Emma K (9)
|
MASSC |
1:06.96 |
1:09.51 |
2.55 |
|
Shaffer, Marissa K (10)
|
MAPCAC |
1:09.52 |
NS |
- |